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Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- NLS4954 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal GPR83 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Human GPR83. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- Reactivity
- Human, Bovine, Porcine, Rabbit, Simian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.05 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Foxp3-dependent and -independent molecules specific for CD25+CD4+ natural regulatory T cells revealed by DNA microarray analysis.
Sugimoto N, Oida T, Hirota K, Nakamura K, Nomura T, Uchiyama T, Sakaguchi S
International immunology 2006 Aug;18(8):1197-209
International immunology 2006 Aug;18(8):1197-209
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GPR83 Antibody [NLS4954] - Analysis of anti-GPR83 antibody with human brain, cortex, neurons at dilution 9-18 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: GPR83 Antibody [NLS4954] - Analysis of anti-GPR83 antibody with purified spleen and lymph nodes stained with GPR83 antibody followed by Alexa488-anti-rabbit IgG. Cells were then stained with APC-anti-CD4 together with PE-anti-CD25 or with PE-anti-FOXP3. Gray is second antibody only and line.
