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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00010241-D01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00010241-D01, RRID:AB_10633399
- Product name
- CALCOCO2 MaxPab rabbit polyclonal antibody (D01)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against a full-length human CALCOCO2 protein.
- Antigen sequence
MEETIKDPPTSAVLLDHCHFSQVIFNSVEKFYIPG
GDVTCHYTFTQHFIPRRKDWIGIFRVGWKTTREYY
TFMWVTLPIDLNNKSAKQQEVQFKAYYLPKDDEYY
QFCYVDEDGVVRGASIPFQFRPENEEDILVVTTQG
EVEEIEQHNKELCKENQELKDSCISLQKQNSDMQA
ELQKKQEELETLQSINKKLELKVKEQKDYWETELL
QLKEQNQKMSSENEKMGIRVDQLQAQLSTQEKEME
KLVQGDQDKTEQLEQLKKENDHLFLSLTEQRKDQK
KLEQTVEQMKQNETTAMKKQQELMDENFDLSKRLS
ENEIICNALQRQKERLEGENDLLKRENSRLLSYMG
LDFNSLPYQVPTSDEGGARQNPGLAYGNPYSGIQE
SSSPSPLSIKKCPICKADDICDHTLEQQQMQPLCF
NCPICDKIFPATEKQIFEDHVFCHSL- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Caspase-11 activation requires lysis of pathogen-containing vacuoles by IFN-induced GTPases.
The ubiquitin ligase parkin mediates resistance to intracellular pathogens.
Meunier E, Dick MS, Dreier RF, Schürmann N, Kenzelmann Broz D, Warming S, Roose-Girma M, Bumann D, Kayagaki N, Takeda K, Yamamoto M, Broz P
Nature 2014 May 15;509(7500):366-70
Nature 2014 May 15;509(7500):366-70
The ubiquitin ligase parkin mediates resistance to intracellular pathogens.
Manzanillo PS, Ayres JS, Watson RO, Collins AC, Souza G, Rae CS, Schneider DS, Nakamura K, Shiloh MU, Cox JS
Nature 2013 Sep 26;501(7468):512-6
Nature 2013 Sep 26;501(7468):512-6
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of CALCOCO2 expression in transfected 293T cell line (H00010241-T02) by CALCOCO2 MaxPab polyclonal antibody.Lane 1: CALCOCO2 transfected lysate(52.30 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of CALCOCO2 transfected lysate using anti-CALCOCO2 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with CALCOCO2 purified MaxPab mouse polyclonal antibody (B01P) (H00010241-B01P).
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
