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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Flow cytometry [2]
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Validation data
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- Product number
- TA501971 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA501971, RRID:AB_11126821
- Product name
- CALCOCO2 mouse monoclonal antibody, clone OTI4H5 (formerly 4H5)
- Antibody type
- Monoclonal
- Description
- CALCOCO2 mouse monoclonal antibody, clone OTI4H5 (formerly 4H5)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- CALCOCO2
- Isotype
- IgG
- Antibody clone number
- OTI4H5
- Vial size
- 100 µl
- Concentration
- NULL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CALCOCO2 (RC203843, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CALCOCO2.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CALCOCO2 monoclonal antibody.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-CALCOCO2 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure from citation: Western Blot of CALCOCO2(NDP52) protein level by using anti-CALCOCO2 antibody in human HEp-2 cell lysates.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-CALCOCO2 mouse monoclonal antibody (TA501971) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CALCOCO2(RC203843).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC203843 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CALCOCO2 antibody(TA501971), and then analyzed by flow cytometry.
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Hela cells, using anti-CALCOCO2 antibody(TA501971),(Red), compared to a nonspecific negative control antibody,(Blue).
- Validation comment
- FC
