12229-1-AP

antibody from Proteintech Group

Targeting: CALCOCO2 MGC17318, NDP52

Provider product page for 12229-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [46]
Comments [0]
Validations
Western blot [2]
Immunoprecipitation [1]
Immunohistochemistry [1]

Submit

Product number: 12229-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#12229-1-AP, RRID:AB_11182600
Product name: NDP52 antibody
Antibody type: Polyclonal
Description: KD/KO validated NDP52 antibody (Cat. #12229-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB, ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

CALCOCO2 protein structure - 12229-1-AP shown in red.

Submitted references USP18 Antagonizes Pyroptosis by Facilitating Selective Autophagic Degradation of Gasdermin D.

Wang L, Li M, Lian G, Yang S, Wu Y, Cui J
Research (Washington, D.C.) 2024;7:0380

Hepatitis B virus core protein as a Rab-GAP suppressor driving liver disease progression.

Su Y, Bu F, Zhu Y, Yang L, Wu Q, Zheng Y, Zhao J, Yu L, Jiang N, Wang Y, Wu J, Xie Y, Zhang X, Gao Y, Lan K, Deng Q
Science bulletin 2024 Apr 8;

Role of Pink1-mediated mitophagy in adenomyosis.

Chen M, Wang W, Fu X, Yi Y, Wang K, Wang M
PeerJ 2023;11:e16497

EGR1 functions as a new host restriction factor for SARS-CoV-2 to inhibit virus replication through the E3 ubiquitin ligase MARCH8.

Zhao Y, Sui L, Wu P, Li L, Liu L, Ma B, Wang W, Chi H, Wang Z-D, Wei Z, Hou Z, Zhang K, Niu J, Jin N, Li C, Zhao J, Wang G, Liu Q
Journal of virology 2023 Oct 31;97(10):e0102823

Modulation of virus-induced neuroinflammation by the autophagy receptor SHISA9 in mice.

Zheng Y, Wang L, Liu Q, Xian H, Zhang C, Cai S, Yang S, Jin S, Cui J
Nature microbiology 2023 May;8(5):958-972

TOLLIP-mediated autophagic degradation pathway links the VCP-TMEM63A-DERL1 signaling axis to triple-negative breast cancer progression.

Zhang TM, Liao L, Yang SY, Huang MY, Zhang YL, Deng L, Hu SY, Yang F, Zhang FL, Shao ZM, Li DQ
Autophagy 2023 Mar;19(3):805-821

VANGL2 inhibits antiviral IFN-I signaling by targeting TBK1 for autophagic degradation.

Hu Z, Xie Y, Lu J, Yang J, Zhang J, Jiang H, Li H, Zhang Y, Wu D, Zeng K, Bai X, Yu X
Science advances 2023 Jun 23;9(25):eadg2339

PRPF19 Limits Porcine Epidemic Diarrhea Virus Replication through Targeting and Degrading Viral Capsid Protein.

Zhai X, Kong N, Wang C, Qin W, Dong S, Zhai H, Yang X, Ye C, Ye M, Li G, Tong W, Liu C, Zheng H, Yu H, Zhang W, Yang X, Tong G, Shan T
Journal of virology 2023 Jan 31;97(1):e0161422

Enhanced liquidity of p62 droplets mediated by Smurf1 links Nrf2 activation and autophagy.

Xia Q, Li Y, Xu W, Wu C, Zheng H, Liu L, Dong L
Cell & bioscience 2023 Feb 21;13(1):37

BNIP3L/NIX-mediated mitophagy alleviates passive stress-coping behaviors induced by tumor necrosis factor-α.

Lu JJ, Wu PF, He JG, Li YK, Long LH, Yao XP, Yang JH, Chen HS, Zhang XN, Hu ZL, Chen Z, Wang F, Chen JG
Molecular psychiatry 2023 Dec;28(12):5062-5076

A potent phosphodiester Keap1-Nrf2 protein-protein interaction inhibitor as the efficient treatment of Alzheimer's disease.

Sun Y, Xu L, Zheng D, Wang J, Liu G, Mo Z, Liu C, Zhang W, Yu J, Xing C, He L, Zhuang C
Redox biology 2023 Aug;64:102793

N protein of PEDV plays chess game with host proteins by selective autophagy.

Zhai X, Kong N, Zhang Y, Song Y, Qin W, Yang X, Ye C, Ye M, Tong W, Liu C, Zheng H, Yu H, Zhang W, Yang X, Zhang G, Tong G, Shan T
Autophagy 2023 Aug;19(8):2338-2352

Isolation of ferret astrocytes reveals their morphological, transcriptional, and functional differences from mouse astrocytes.

Roboon J, Hattori T, Nguyen DT, Ishii H, Takarada-Iemata M, Kannon T, Hosomichi K, Maejima T, Saito K, Shinmyo Y, Mieda M, Tajima A, Kawasaki H, Hori O
Frontiers in cellular neuroscience 2022;16:877131

Therapeutic effects of total saikosaponins from Radix bupleuri against Alzheimer's disease.

Li J, Zou B, Cheng XY, Yang XH, Li J, Zhao CH, Ma RX, Tian JX, Yao Y
Frontiers in pharmacology 2022;13:940999

Autophagy Activation Induces p62-Dependent Autophagic Degradation of Dengue Virus Capsid Protein During Infection.

Wu Y, Zhou T, Hu J, Liu Y, Jin S, Wu J, Guan X, Cui J
Frontiers in microbiology 2022;13:889693

Deficiency of the frontotemporal dementia gene GRN results in gangliosidosis.

Boland S, Swarup S, Ambaw YA, Malia PC, Richards RC, Fischer AW, Singh S, Aggarwal G, Spina S, Nana AL, Grinberg LT, Seeley WW, Surma MA, Klose C, Paulo JA, Nguyen AD, Harper JW, Walther TC, Farese RV Jr
Nature communications 2022 Oct 7;13(1):5924

TARDBP Inhibits Porcine Epidemic Diarrhea Virus Replication through Degrading Viral Nucleocapsid Protein and Activating Type I Interferon Signaling.

Dong S, Kong N, Zhang Y, Li Y, Sun D, Qin W, Zhai H, Zhai X, Yang X, Ye C, Ye M, Liu C, Yu L, Zheng H, Tong W, Yu H, Zhang W, Tong G, Shan T
Journal of virology 2022 May 25;96(10):e0007022

Panax notoginseng saponins protect PC12 cells against Aβ induced injury via promoting parkin-mediated mitophagy.

Jiang Y, Li H, Huang P, Li S, Li B, Huo L, Zhong J, Pan Z, Li Y, Xia X
Journal of ethnopharmacology 2022 Mar 1;285:114859

FUBP3 Degrades the Porcine Epidemic Diarrhea Virus Nucleocapsid Protein and Induces the Production of Type I Interferon.

Dong S, Kong N, Wang C, Li Y, Sun D, Qin W, Zhai H, Zhai X, Yang X, Ye C, Ye M, Liu C, Yu L, Zheng H, Tong W, Yu H, Zhang W, Tong G, Shan T
Journal of virology 2022 Jul 13;96(13):e0061822

Selective autophagy controls the stability of TBK1 via NEDD4 to balance host defense.

Xie W, Jin S, Zhang C, Yang S, Wu Y, Zhao Y, Songyang Z, Cui J
Cell death and differentiation 2022 Jan;29(1):40-53

A detrimental role of NLRP6 in host iron metabolism during Salmonella infection.

Deng Q, Yang S, Sun L, Huang K, Dong K, Zhu Y, Cao Y, Li Y, Wu S, Huang R
Redox biology 2022 Feb;49:102217

TRIM14 inhibits OPTN-mediated autophagic degradation of KDM4D to epigenetically regulate inflammation.

Liu D, Zhao Z, She Y, Zhang L, Chen X, Ma L, Cui J
Proceedings of the National Academy of Sciences of the United States of America 2022 Feb 15;119(7)

Autophagy receptor OPTN (optineurin) regulates mesenchymal stem cell fate and bone-fat balance during aging by clearing FABP3.

Liu ZZ, Hong CG, Hu WB, Chen ML, Duan R, Li HM, Yue T, Cao J, Wang ZX, Chen CY, Hu XK, Wu B, Liu HM, Tan YJ, Liu JH, Luo ZW, Zhang Y, Rao SS, Luo MJ, Yin H, Wang YY, Xia K, Xu L, Tang SY, Hu RG, Xie H
Autophagy 2021 Oct;17(10):2766-2782

PINK1-mediated mitophagy maintains pluripotency through optineurin.

Wang C, Liu K, Cao J, Wang L, Zhao Q, Li Z, Zhang H, Chen Q, Zhao T
Cell proliferation 2021 May;54(5):e13034

TDP-43 and PINK1 mediate CHCHD10(S59L) mutation-induced defects in Drosophila and in vitro.

Baek M, Choe YJ, Bannwarth S, Kim J, Maitra S, Dorn GW 2nd, Taylor JP, Paquis-Flucklinger V, Kim NC
Nature communications 2021 Mar 26;12(1):1924

Galectin-9 Targets NLRP3 for Autophagic Degradation to Limit Inflammation.

Wang W, Qin Y, Song H, Wang L, Jia M, Zhao C, Gong M, Zhao W
Journal of immunology (Baltimore, Md. : 1950) 2021 Jun 1;206(11):2692-2699

FIP200 controls the TBK1 activation threshold at SQSTM1/p62-positive condensates.

Schlütermann D, Berleth N, Deitersen J, Wallot-Hieke N, Friesen O, Wu W, Stuhldreier F, Sun Y, Berning L, Friedrich A, Mendiburo MJ, Peter C, Wiek C, Hanenberg H, Stefanski A, Stühler K, Stork B
Scientific reports 2021 Jul 5;11(1):13863

Oxidative stress-induced mitophagy is suppressed by the miR-106b-93-25 cluster in a protective manner.

Zhang C, Nie P, Zhou C, Hu Y, Duan S, Gu M, Jiang D, Wang Y, Deng Z, Chen J, Chen S, Wang L
Cell death & disease 2021 Feb 24;12(2):209

Nitazoxanide impairs mitophagy flux through ROS-mediated mitophagy initiation and lysosomal dysfunction in bladder cancer.

Sun H, Ou T, Hu J, Yang Z, Lei Q, Li Y, Wang G, Li Y, Wu K, Wang S, Wu S
Biochemical pharmacology 2021 Aug;190:114588

METTL14 facilitates global genome repair and suppresses skin tumorigenesis.

Yang Z, Yang S, Cui YH, Wei J, Shah P, Park G, Cui X, He C, He YY
Proceedings of the National Academy of Sciences of the United States of America 2021 Aug 31;118(35)

CNTNAP4 deficiency in dopaminergic neurons initiates parkinsonian phenotypes.

Zhang W, Zhou M, Lu W, Gong J, Gao F, Li Y, Xu X, Lin Y, Zhang X, Ding L, Zhang Z, Li G, Chen X, Sun X, Zhu X, Xu P, Zhang Y
Theranostics 2020;10(7):3000-3021

ISG15 Connects Autophagy and IFN-γ-Dependent Control of Toxoplasma gondii Infection in Human Cells.

Bhushan J, Radke JB, Perng YC, Mcallaster M, Lenschow DJ, Virgin HW, Sibley LD
mBio 2020 Oct 6;11(5)

ALS-FTLD-linked mutations of SQSTM1/p62 disrupt selective autophagy and NFE2L2/NRF2 anti-oxidative stress pathway.

Deng Z, Lim J, Wang Q, Purtell K, Wu S, Palomo GM, Tan H, Manfredi G, Zhao Y, Peng J, Hu B, Chen S, Yue Z
Autophagy 2020 May;16(5):917-931

Microglia clear neuron-released α-synuclein via selective autophagy and prevent neurodegeneration.

Choi I, Zhang Y, Seegobin SP, Pruvost M, Wang Q, Purtell K, Zhang B, Yue Z
Nature communications 2020 Mar 13;11(1):1386

Streptococcus pneumoniae triggers hierarchical autophagy through reprogramming of LAPosome-like vesicles via NDP52-delocalization.

Ogawa M, Takada N, Shizukuishi S, Tomokiyo M, Chang B, Yoshida M, Kakuta S, Tanida I, Ryo A, Guan JL, Takeyama H, Ohnishi M
Communications biology 2020 Jan 13;3(1):25

GABARAPL2 Is Critical for Growth Restriction of Toxoplasma gondii in HeLa Cells Treated with Gamma Interferon.

Zhang Z, Gu H, Li Q, Zheng J, Cao S, Weng C, Jia H
Infection and immunity 2020 Apr 20;88(5)

Proteasome inhibition boosts autophagic degradation of ubiquitinated-AGR2 and enhances the antitumor efficiency of bevacizumab.

Wang D, Xu Q, Yuan Q, Jia M, Niu H, Liu X, Zhang J, Young CY, Yuan H
Oncogene 2019 May;38(18):3458-3474

TEX264 Is an Endoplasmic Reticulum-Resident ATG8-Interacting Protein Critical for ER Remodeling during Nutrient Stress.

An H, Ordureau A, Paulo JA, Shoemaker CJ, Denic V, Harper JW
Molecular cell 2019 Jun 6;74(5):891-908.e10

Myocardial hypothermia increases autophagic flux, mitochondrial mass and myocardial function after ischemia-reperfusion injury.

Marek-Iannucci S, Thomas A, Hou J, Crupi A, Sin J, Taylor DJ, Czer LS, Esmailian F, Mentzer RM Jr, Andres AM, Gottlieb RA
Scientific reports 2019 Jul 10;9(1):10001

A selective inhibitor of mitofusin 1-βIIPKC association improves heart failure outcome in rats.

Ferreira JCB, Campos JC, Qvit N, Qi X, Bozi LHM, Bechara LRG, Lima VM, Queliconi BB, Disatnik MH, Dourado PMM, Kowaltowski AJ, Mochly-Rosen D
Nature communications 2019 Jan 18;10(1):329

Beclin1-driven autophagy modulates the inflammatory response of microglia via NLRP3.

Houtman J, Freitag K, Gimber N, Schmoranzer J, Heppner FL, Jendrach M
The EMBO journal 2019 Feb 15;38(4)

Activation of PINK1-Parkin-dependent mitophagy in Tri-ortho-cresyl phosphate-treated Neuro2a cells.

Wang Y, Zhang C, Shen Z, Kou R, Xie K, Song F
Chemico-biological interactions 2019 Aug 1;308:70-79

Tetherin Suppresses Type I Interferon Signaling by Targeting MAVS for NDP52-Mediated Selective Autophagic Degradation in Human Cells.

Jin S, Tian S, Luo M, Xie W, Liu T, Duan T, Wu Y, Cui J
Molecular cell 2017 Oct 19;68(2):308-322.e4

NF-κB Restricts Inflammasome Activation via Elimination of Damaged Mitochondria.

Zhong Z, Umemura A, Sanchez-Lopez E, Liang S, Shalapour S, Wong J, He F, Boassa D, Perkins G, Ali SR, McGeough MD, Ellisman MH, Seki E, Gustafsson AB, Hoffman HM, Diaz-Meco MT, Moscat J, Karin M
Cell 2016 Feb 25;164(5):896-910

Phosphatase and tensin homologue (PTEN)-induced putative kinase 1 reduces pancreatic β-cells apoptosis in glucotoxicity through activation of autophagy.

Zhang J, Chen K, Wang L, Wan X, Shrestha C, Zhou J, Mo Z
Biochemical and biophysical research communications 2016 Aug 5;476(4):299-305

A Noncanonical Autophagy Pathway Restricts Toxoplasma gondii Growth in a Strain-Specific Manner in IFN-γ-Activated Human Cells.

Selleck EM, Orchard RC, Lassen KG, Beatty WL, Xavier RJ, Levine B, Virgin HW, Sibley LD
mBio 2015 Sep 8;6(5):e01157-15

No comments: Submit comment

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HeLa cells were subjected to SDS PAGE followed by western blot with 12229-1-AP(CALCOCO2 antibody) at dilution of 1:1000
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12229-1-AP( CALCOCO2 Antibody) at dilution of 1:1000
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: IP Result of anti-CALCOCO2 (IP:12229-1-AP, 3ug; Detection:12229-1-AP 1:1000) with HeLa cells lysate 2150ug.
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The CALCOCO2 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human CALCOCO2. This antibody recognizes human, mouse, rat antigen. The CALCOCO2 antibody has been validated for the following applications: ELISA, WB, IHC, IP analysis.
Appendix: 12229-1-AP.pdf

: Show more