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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [5]
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- Product number
- PA5-52293 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GRHL1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: VTVSIATMPT HSIKTETQPH GFAVGIPPAV YHPEPTERVV VFDRNLNTDQ FSSGAQAPNA QRRTPDSTFS ETFKEGVQEV FFPSDLSLRM PGMNSEDYVF DSVSGNNFEY TLEASKSLRQ KPGDSTMTYL NK
- Concentration
- 0.1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GRHL1 in control (vector only transfected HEK293T lysate) and GRHL1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a GRHL1 Polyclonal Antibody (Product # PA5-52293).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of GRHL1 in human cell line U-2 OS shows positivity in vesicles & nucleus but excluded from the nucleoli. Samples were probed using a GRHL1 Polyclonal Antibody (Product # PA5-52293).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of GRHL1 in human skin and skeletal muscle tissues using GRHL1 Polyclonal Antibody (Product # PA5-52293). Corresponding GRHL1 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of GRHL1 in human placenta using GRHL1 Polyclonal Antibody (Product # PA5-52293) shows strong nuclear positivity in trophoblastic cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of GRHL1 in human tonsil using GRHL1 Polyclonal Antibody (Product # PA5-52293) shows moderate nuclear positivity in squamous epithelial cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of GRHL1 in human skin using GRHL1 Polyclonal Antibody (Product # PA5-52293) shows moderate to strong nuclear positivity in a subset of squamous epithelial cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of GRHL1 in human skeletal muscle using GRHL1 Polyclonal Antibody (Product # PA5-52293) shows no positivity in myocytes as expected.
