Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Chromatin Immunoprecipitation [1]
- Other assay [1]
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Validation data
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- Product number
- 711087 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TBX3 Recombinant Polyclonal Antibody (22HCLC)
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 22HCLC
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Establishment of the TBX-code reveals aberrantly activated T-box gene TBX3 in Hodgkin lymphoma.
Nagel S, Meyer C
PloS one 2021;16(11):e0259674
PloS one 2021;16(11):e0259674
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- For immunofluorescence analysis, MCF7 cells were fixed and permeabilized for detection of endogenous TBX3 using Anti- TBX3 Recombinant Rabbit Polyclonal Antibody (Product # 711087, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of TBX3 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating nuclear localization of TBX3. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Enrichment of endogenous TBX3 protein at specific gene loci using Anti-TBX3 Recombinant Rabbit Polyclonal Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-TBX3 Recombinant Rabbit Monoclonal Antibody (Product # 711087, 5 µg) on sheared chromatin from 2 million HEPG2 cells using the "MAGnify ChIP system" kit (Product # 49-2024). Normal Rabbit IgG (1 µg) was used as a negative IP control. The purified DNA was analyzed by 7500 Fast qPCR system (Product # 4351106) with optimized PCR primer pairs for the different loci of the active P21 promoter region used as positive control target genes, and the region of the inactive MYOD, SAT2 satellite repeat, used as negative control target gene. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 10.1371/journal.pone.0259674.g002 Fig 2 TBX3 and TBX5 expression in HL cell lines. (A) RNA-seq data show expression levels of TBX3 in 100 leukemia/lymphoma cell lines using dataset E-MTAB-7721. Note the singularly high transcript levels in HL cell line KM-H2. HL cell lines are indicated. (B) RQ-PCR analysis of TBX3 in HL cell lines, confirming elevated expression in KM-H2. (C) RQ-PCR analysis of TBX3 in KM-H2 in comparison to primary samples derived from B-cells, T-cells, spleen, lung and retina. (D) Western blot analysis of TBX3 in HL cell lines shows TBX3 expression at the protein level in KM-H2. (E) Immuno-fluorescence microscopy of KM-H2 (above) and L-428 cells (below) using TBX3 antibody (green) and nuclear counterstain DAPI (blue).