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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- GTX80913 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX80913, RRID:AB_11165719
- Product name
- EMX1 antibody, C-term
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- EMX1 Antibody (C-term) (GTX80913) western blot analysis in U251 and Neuro-2a cell line mouse cerebellum tissue lysates (35ug/lane).This demonstrates the EMX1 antibody detected the EMX1 protein (arrow).
- Validation comment
- WB
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Fluorescent confocal image of Hela cell stained with EMX1 Antibody (C-term)(GTX80913).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with EMX1 primary antibody (1:25, 1 h at 37¢J). For secondary antibody, Alexa Fluor? 488 conjμgated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37¢J).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjμgated Phalloidin (7units/ml, 1 h at 37¢J). Nuclei were counterstained with DAPI (blue) (10 μg/ml, 10 min). EMX1 immunoreactivity is localized to Nucleus significantly.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- EMX1 Antibody (C-term) (GTX80913) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjμgated goat-anti-rabbit secondary antibodies were used for the analysis.
