PA5-51036

antibody from Invitrogen Antibodies

Targeting: PTGES MGST-IV, MGST1-L1, MGST1L1, PIG12, TP53I12

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Antibody Data

Product number

PA5-51036

Provider

Invitrogen Antibodies

Product name

PTGES Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Small Molecule Interactome Mapping by Photo-Affinity Labeling (SIM-PAL) to Identify Binding Sites of Small Molecules on a Proteome-Wide Scale.

Flaxman HA, Miyamoto DK, Woo CM
Current protocols in chemical biology 2019 Dec;11(4):e75

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PTGES in A549 cells. Samples were probed using PTGES Polyclonal Antibody (Product # PA5-51036).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-PTGES Polyclonal Antibody(Product # PA5-51036) and a 18kDa band corresponding to PTGES was observed across positive cell lines tested. Membrane enriched extracts (30 µg lysate) of A-431 (Lane 1), DU 145 (Lane 2) and BeWo (Lane 3) (positive cell lines); and HEL 92.1.7 (Lane 4), LNCaP (Lane 5) and PC-3 (Lane 6) (negative cell lines) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0341BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).