PA5-60916

antibody from Invitrogen Antibodies

Targeting: PTGES MGST-IV, MGST1-L1, MGST1L1, PIG12, TP53I12

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-60916

Provider

Invitrogen Antibodies

Product name

PTGES Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: ITGQVRLRKK AFANPEDALR HGGPQYCRSD PDVERCLRAP RNDM

Concentration

0.13 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PTGES in human cell lines A-549 and U-251MG using a PTGES Polyclonal Antibody (Product # PA5-60916). Corresponding PTGES RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PTGES in mouse cell line NIH-3T3 and rat cell line NBT-II using a PTGES Polyclonal Antibody (Product # PA5-60916).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-PTGES Polyclonal Antibody (Product # PA5-60916) and an 18 kDa band corresponding to PTGES was observed across positive cell lines (A-431, BeWo and DU 145); and not in negative cell lines (LNCaP, PC-3 and SH-SY5Y). Whole cell extracts (30 µg lysate) of A-431 (Lane 1), BeWo (Lane 2), DU 145 (Lane 3), LNCaP (Lane 4), PC-3 (Lane 5) and SH-SY5Y (Lane 6) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0341BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.1 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of PTGES in human cell line SiHa using a PTGES Polyclonal Antibody (Product # PA5-60916) shows localization to endoplasmic reticulum.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of PTGES in human seminal vesicle and endometrium tissues using PTGES Polyclonal Antibody (Product # PA5-60916). Corresponding PTGES RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of PTGES in human seminal vesicle using PTGES Polyclonal Antibody (Product # PA5-60916) shows high expression.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of PTGES in human endometrium using PTGES Polyclonal Antibody (Product # PA5-60916) shows low expression as expected.