91369ACTMOTIF

antibody from Invitrogen Antibodies

Targeting: KDM4D FLJ10251, JMJD2D

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

91369ACTMOTIF

Provider

Invitrogen Antibodies

Product name

JMJD2D/KDM4D Recombinant Mouse Monoclonal Antibody, AbFlex®

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

AbFlex JMJD2D/KDM4D antibody tested by Western blot. 20 µg of HEK293 nuclear extract was run on SDS-PAGE and probed with antibody at 0.5 µg/mL.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

AbFlex JMJD2D/KDM4D antibody tested by Dot blot. Recombinant KDM proteins were spotted onto nitrocellulose membrane (nanograms indicated on row labels) and probed with antibody at 1 µg/mL. Col. 1: KDM4A. Col. 2: KDM4B. Col. 3: KDM4C. Col. 4: KDM5A. Col. 5: KDM5B. Col. 6: KDM5C.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

AbFlex JMJD2D/KDM4D antibody tested by Immunoprecipitation. 4 µg of AbFlex JMJD2D/KDM4 antibody (Lane1) or isotype control mouse IgG (Lane 2) was used to immunoprecipitate JMJD2D/KDM4D from 500 µg of HEK293 extract, which was then run on SDS-PAGE and probed with the same antibody at 2 µg/mL. The appearance of antibody light chain at 25 kDa is observed as a cross-reactivity between the WB / IP antibody, and is common.