Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [3]
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Validation data
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- Product number
- NBP2-19586 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal Nrf1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Western Blot: Nrf1 Antibody [NBP2-19586] - A. 30 ug 293T whole cell lysate/extract B. 30 ug 293T nuclear lysate/extract C. 30 ug A431 whole cell lysate/extract | D. 30 ug A431 nuclear lysate/extract E. 30 ug HeLa whole cell lysate/extract F. 30 ug HeLa nuclear lysate/extract G. 30 ug HepG2 whole cell lysate/extract H. 30 ug HepG2 nuclear lysate/extract 10 % SDS-PAGE NRF1 antibody dilution: 1:1000
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Nrf1 Antibody [NBP2-19586] - IP samples: 293T whole cell extract A. Control with 4 ug of preimmune Rabbit IgG B. Immunoprecipitation of NRF1 protein by 4 ug NRF1 antibody. 10 % SDS-PAGE The immunoprecipitated NRF1 protein was detected by NRF1 antibody diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent].
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Nrf1 Antibody [NBP2-19586] - Paraffin-embedded human lung adenocarcinoma. NRF1 antibody diluted at 1:250.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Nrf1 Antibody [NBP2-19586] - Paraffin-embedded mouse muscle. NRF1 antibody dilution: 1:500.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Nrf1 Antibody [NBP2-19586] - Paraffin-embedded mouse colon. NRF1 antibody dilution: 1:500.