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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- MAB5306 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Nrf1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects endogenous human, mouse and rat Nrf1 at 85 kDa in Western blots.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Protective effects of myricetin on acute hypoxia-induced exercise intolerance and mitochondrial impairments in rats.
Zou D, Liu P, Chen K, Xie Q, Liang X, Bai Q, Zhou Q, Liu K, Zhang T, Zhu J, Mi M
PloS one 2015;10(4):e0124727
PloS one 2015;10(4):e0124727
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Detection of Human, Mouse, and Rat Nrf1 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, MOLT-4 human acute lymphoblastic leukemia cell line, SH-SY5Y human neuroblastoma cell line, A20 mouse B cell lymphoma cell line, and Y3-Ag rat myeloid cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat Nrf1 Monoclonal Antibody (Catalog # MAB5306) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Nrf1 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
