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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-21806 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- C9orf78 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji, Neuro 2A, C8D30, NIH-3T3, Raw264.7, C2C12, PC-12, Rat2.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of C9orf78 was performed by separating 30 µg of various whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a C9orf78 Polyclonal Antibody (Product # PA5-21806) at a dilution of 1:500 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of C9orf78 was performed by separating 30 µg of various whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a C9orf78 Polyclonal Antibody (Product # PA5-21806) at a dilution of 1:500 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using C9orf78 Polyclonal Antibody (Product # PA5-21806). Sample (30 µg whole cell lysate). A: H1299. B: HeLa S3. C: Hep G2. 12% SDS PAGE. C9orf78 Polyclonal Antibody (Product # PA5-21806) diluted at 1:3,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of C9orf78 was performed by separating 30 µg of various whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a C9orf78 Polyclonal Antibody (Product # PA5-21806) at a dilution of 1:500 and a HRP-conjugated anti-rabbit IgG secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of C9orf78 in paraformaldehyde-fixed HeLa cells using a C9orf78 polyclonal antibody (Product # PA5-21806) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using C9orf78 antibody (Product # PA5-21806) at 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using C9orf78 (Product # PA5-21806) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
