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- Product number
- NBP1-83169 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-83169, RRID:AB_11059253
- Product name
- Rabbit Polyclonal HCA59 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human HCA59 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Contribution of antibody-based protein profiling to the human Chromosome-centric Proteome Project (C-HPP).
Fagerberg L, Oksvold P, Skogs M, Algenäs C, Lundberg E, Pontén F, Sivertsson A, Odeberg J, Klevebring D, Kampf C, Asplund A, Sjöstedt E, Al-Khalili Szigyarto C, Edqvist PH, Olsson I, Rydberg U, Hudson P, Ottosson Takanen J, Berling H, Björling L, Tegel H, Rockberg J, Nilsson P, Navani S, Jirström K, Mulder J, Schwenk JM, Zwahlen M, Hober S, Forsberg M, von Feilitzen K, Uhlén M
Journal of proteome research 2013 Jun 7;12(6):2439-48
Journal of proteome research 2013 Jun 7;12(6):2439-48
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HCA59 Antibody [NBP1-83169] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-C9orf78 antibody. Remaining relative intensity is presented.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HCA59 Antibody [NBP1-83169] - Analysis in control (vector only transfected HEK293T lysate) and C9orf78 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83169] - Staining of human epididymis shows strong nuclear positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83169] - Staining of human bone marrow shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83169] - Staining of human pancreas shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83169] - Staining of human testis.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83169] - Staining of human lymph node.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83169] - Staining of human kidney.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: HCA59 Antibody [NBP1-83169] - Staining of human Fallopian tube shows moderate nuclear positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83169] - Analysis in human bone marrow and pancreas tissues. Corresponding HCA59 RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83169] - Staining of human bone marrow, fallopian tube, lymph node and testis using Anti-HCA59 antibody NBP1-83169 (A) shows similar protein distribution across tissues to independent antibody NBP1-83170 (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83169] - Staining of human lymph node shows strong nuclear positivity in non-germinal center cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83169] - Staining of human pancreas shows weak positivity in exocrine glandular cells as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83169] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
