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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
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Validation data
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- Product number
- 25539-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PGBD1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: CVKTYPLES GEEAVTVLEN LETGSGDTGQ QASVYIQGQD MHPMVAEYQG VSLECQSLQL LPGITTLKCE PPQRPQGNPQ EVSGPVPHGS AHLQEKNPRD KAVVPVFNPV RSQTLVKTEE ETAQAVAAEK WSHLSLTRRN LCGNSAQETV MSLSPMTEEI VTKDRLFKAK QETSEEMEQS GEASGKPNRE CAPQIPCSTP IATERTVAHL NTLKDRHPGD LWARMHISSL EYAAGDITRK GRKKDKARVS ELLQGLSFSG DSDVEKDNEP EIQPAQKKLK VSCFPEKSWT KRDIKPNFPS WSALDSGLLN LKSEKLNPVE LFELFFDDET FNLIVNETNN YASQKNVSLE VTV (103-454 aa encoded by BC128585)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.27 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 25539-1-AP ( PGBD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- K-562 cells were subjected to SDS PAGE followed by western blot with 25539-1-AP (PGBD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells using 25539-1-AP (PGBD1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney tissue slide using 25539-1-AP (PGBD1 Antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis tissue slide using 25539-1-AP (PGBD1 Antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney tissue slide using 25539-1-AP ( PGBD1 Antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis tissue slide using 25539-1-AP ( PGBD1 Antibody) at dilution of 1:50 (under 40x lens).
