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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- PAB10228 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10228, RRID:AB_1671690
- Product name
- CBX4 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of CBX4.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references The polycomb protein Pc2 is a SUMO E3.
Interference with the expression of a novel human polycomb protein, hPc2, results in cellular transformation and apoptosis.
RING1 is associated with the polycomb group protein complex and acts as a transcriptional repressor.
Kagey MH, Melhuish TA, Wotton D
Cell 2003 Apr 4;113(1):127-37
Cell 2003 Apr 4;113(1):127-37
Interference with the expression of a novel human polycomb protein, hPc2, results in cellular transformation and apoptosis.
Satijn DP, Olson DJ, van der Vlag J, Hamer KM, Lambrechts C, Masselink H, Gunster MJ, Sewalt RG, van Driel R, Otte AP
Molecular and cellular biology 1997 Oct;17(10):6076-86
Molecular and cellular biology 1997 Oct;17(10):6076-86
RING1 is associated with the polycomb group protein complex and acts as a transcriptional repressor.
Satijn DP, Gunster MJ, van der Vlag J, Hamer KM, Schul W, Alkema MJ, Saurin AJ, Freemont PS, van Driel R, Otte AP
Molecular and cellular biology 1997 Jul;17(7):4105-13
Molecular and cellular biology 1997 Jul;17(7):4105-13
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blotting analysis shows the detection of human CBX4 in probed lysates using CBX4 polyclonal antibody (Cat # PAB10228).The panel on the left shows the blot probed with CBX4 polyclonal antibody.The panel on the right is the same blot reprobed with anti-FLAG antibody to confirm the presence of FLAG tagged recombinant CBX4 in the lysate.In the left panel the band labeled as CBX4 is FLAG-tagged transfected CBX4 in 293T cells.The bands labeled with stars are likely endogenous CBX4.In the right panel the band labeled CBX4 is FLAG-tagged transfected CBX4 in 293T cells.Data contributed by Dr. Ari Melnick, Albert Einstein College of Medicine.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence microscopy of CBX4 polyclonal antibody (Cat # PAB10228) was used for immunofluorescent imaging of U-2 OS.The image reveals the expected discrete nuclear structure that is termed the PcG body corresponding to the known localization of CBX4 (see Satijn et al. below) .Primary antibody used at 1 : 200.Data contributed by Luke Hughes-Davies and Rhiannon Jade, Gurdon Institute, Cambridge, UK.
- Validation comment
- Immunofluorescence
