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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- GTX24189 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX24189, RRID:AB_381301
- Product name
- CBX4 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blotting. Analysis shows the detection of human PC2 in probed lysates using GeneTex Affinity Purified anti-hPC2 antibody (GTX24189). The panel on the left shows the blot probed with anti-hPC2. The panel on the right is the same blot reprobed with anti-FLAG antibody to confirm the presence of FLAG tagged recombinant PC2 in the lysate. In the left panel the band labeled as hPC2 is FLAG-tagged transfected hPC2 in 293T cells. The bands labeled with stars are likely endogenous hPC2. In the right panel the band labeled hPC2 is FLAG-tagged transfected hPC2 in 293T cells. Data contributed by Dr Ari Melnick, Albert Einstein College of Medicine.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blotting. Analysis shows the detection of human PC2 in probed lysates using GeneTex Affinity Purified anti-hPC2 antibody (GTX24189). The panel on the left shows the blot probed with anti-hPC2. The panel on the right is the same blot reprobed with anti-FLAG antibody to confirm the presence of FLAG tagged recombinant PC2 in the lysate. In the left panel the band labeled as hPC2 is FLAG-tagged transfected hPC2 in 293T cells. The bands labeled with stars are likely endogenous hPC2. In the right panel the band labeled hPC2 is FLAG-tagged transfected hPC2 in 293T cells.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GeneTex anti-hPC2 antibody (GTX24189) was used for immunofluorescent imaging of human cells (U2OS). The image reveals the expected discrete nuclear structure that is termed the PcG body corresponding to the known localization of PC2. IF was performed after fixation in PBS with 4% PF for 5 min, permeabilization with 0.5% Polyethylene glycol tert-octylphenyl ether-PBS for 5 min, and blocking with 5% milk / 0.2% Tween for 1 h. Primary antibody used at 1:200 in 5% milk / 0.2% Tween for 1 h, secondary antibody for 30 min. All blocking and incubation steps carried out at 37¢X C. Nuclei were counterstained with Hoechst stain (blue).
