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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- PA5-20256 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TRPC6 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is mouse lung tissue lysate. PA5-20256 can be used with blocking peptide PEP-0392.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Submitted references Myocardial Hypertrophy and Fibrosis Are Associated with Cardiomyocyte Beta-Catenin and TRPC6/Calcineurin/NFAT Signaling in Spontaneously Hypertensive Rats with 5/6 Nephrectomy.
Bogdanova E, Beresneva O, Galkina O, Zubina I, Ivanova G, Parastaeva M, Semenova N, Dobronravov V
International journal of molecular sciences 2021 Apr 28;22(9)
International journal of molecular sciences 2021 Apr 28;22(9)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of mouse lung tissue lysate using a TRPC6 polyclonal antibody (Product # PA5-20256) at (A) 0.5, (B) 1 and (C) 2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of TRPC6 in mouse lung tissue lysate with TRPC6 Polyclonal Antibody (Product # PA5-20256) at (A) 0.5, (B) 1 and (C) 2 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of mouse lung cells using a TRPC6 polyclonal antibody (Product # PA5-20256) at a 20 µg/mL dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of TRPC6 in Mouse Lung cells with TRPC6 Polyclonal Antibody (Product # PA5-20256) at 20 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of TRPC6 in mouse lung tissue with TRPC6 Polyclonal Antibody (Product # PA5-20256) at 10 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of TRPC6 in mouse lung tissue with TRPC6 Polyclonal Antibody (Product # PA5-20256) at 5 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 Myocardial expression of calcineurin/NFAT and TRPC6: ( a ) the representative microphotographs of the myocardial expression of TRPC6/CaN/NFAT; the morphometric analysis of myocardial ( b ) TRPC6 (200 x ; scale bar, 100 mum), ( c ) CaN (200 x ), and ( d ) nuclear NFATc3 (400 x ; scale bar, 50 mum) IHC staining; and ( c ) the relative mRNA expression of ( e ) Trpc6 , ( f ) calcineurin A ( Ppp3ca ) and ( g ) calcineurin B ( Ppp3r1 ) in controls, hypertension, and renal dysfunction; FOV--field of view; Nu--nuclei.
