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Western blot
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- Antibody Data
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- References [1]
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- Validations
- Western blot [1]
- Flow cytometry [1]
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- Product number
- NB600-791 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-791, RRID:AB_2208350
- Product name
- Goat Polyclonal TRPC6 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references A mutation in the TRPC6 cation channel causes familial focal segmental glomerulosclerosis.
Winn MP, Conlon PJ, Lynn KL, Farrington MK, Creazzo T, Hawkins AF, Daskalakis N, Kwan SY, Ebersviller S, Burchette JL, Pericak-Vance MA, Howell DN, Vance JM, Rosenberg PB
Science (New York, N.Y.) 2005 Jun 17;308(5729):1801-4
Science (New York, N.Y.) 2005 Jun 17;308(5729):1801-4
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TRPC6 Antibody [NB600-791] - Staining of A549 cell lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TRPC6 Antibody [NB600-791] - Analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
