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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
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- Product number
- PA1-31950 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-FOXL2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: The peptide used to generate this antibody is available for purchase (GTX25096-PEP).. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Oleic acid induces down-regulation of the granulosa cell identity marker FOXL2, and up-regulation of the Sertoli cell marker SOX9 in bovine granulosa cells.
Yenuganti VR, Vanselow J
Reproductive biology and endocrinology : RB&E 2017 Jul 26;15(1):57
Reproductive biology and endocrinology : RB&E 2017 Jul 26;15(1):57
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FOXL2 in human Heart lysate (35 µg protein in RIPA buffer) using a FOXL2 polyclonal antibody (Product # PA1-31950) at a dilution of 0.5 µg/mL following incubation for 1 hour and detected using chemiluminescence.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FOXL2 in human Ovary lysate (35 µg protein in RIPA buffer) using a FOXL2 polyclonal antibody (Product # PA1-31950) at a dilution of 1 µg/mL following incubation for 1 hour and detected using chemiluminescence.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of FOXL2 was performed by loading 35 µg (in RIPA buffer) of human (A) and mouse (B) ovary lysates. Proteins were transferred to a membrane and probed with a FOXL2 Polyclonal Antibody (Product # PA1-31950) at a dilution of 1 µg/mL.
