LS-C185626

antibody from LSBio

Targeting: LDB1 CLIM2, NLI

Western blot

Antibody data

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Validation data

Product number: LS-C185626 - Provider product page
Provider: LSBio
Product name: LDB1 / CLIM2 Antibody (aa86-411) LS-C185626
Antibody type: Polyclonal
Description: Immunoaffinity purified
Reactivity: Human, Mouse
Host: Rabbit
Isotype: IgG
Storage: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

LDB1 protein structure - LS-C185626 shown in red.

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: LDB1antibody immunoprecipitates LDB1 protein in IP experiments. IP Sample:1000 ug 293T whole cell lysate/extract. A. 20 ug 293T whole cell lysate/extract. B. Control with 2.5 ug of preimmune rabbit IgG. C. Immunoprecipitation of LDB1 protein by 2.5 ug of LDB1 antibody . 12% SDS-PAGE. The immunoprecipitated LDB1 protein was detected by LDB1antibody diluted at 1:1000. EasyBlot anti-rabbit IgG (anti-rabbit IgG (HRP) -01) was used as a secondary reagent.

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: LDB1antibody immunoprecipitates LDB1 protein in IP experiments. IP Sample:1000 ug 293T whole cell lysate/extract. A. 20 ug 293T whole cell lysate/extract. B. Control with 2.5 ug of preimmune rabbit IgG. C. Immunoprecipitation of LDB1 protein by 2.5 ug of LDB1 antibody . 12% SDS-PAGE. The immunoprecipitated LDB1 protein was detected by LDB1antibody diluted at 1:1000. EasyBlot anti-rabbit IgG (anti-rabbit IgG (HRP) -01) was used as a secondary reagent.

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: LDB1antibody immunoprecipitates LDB1 protein in IP experiments. IP Sample:1000 ug 293T whole cell lysate/extract. A. 20 ug 293T whole cell lysate/extract. B. Control with 2.5 ug of preimmune rabbit IgG. C. Immunoprecipitation of LDB1 protein by 2.5 ug of LDB1 antibody . 12% SDS-PAGE. The immunoprecipitated LDB1 protein was detected by LDB1antibody diluted at 1:1000. EasyBlot anti-rabbit IgG (anti-rabbit IgG (HRP) -01) was used as a secondary reagent.

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: LDB1antibody immunoprecipitates LDB1 protein in IP experiments. IP Sample:1000 ug 293T whole cell lysate/extract. A. 20 ug 293T whole cell lysate/extract. B. Control with 2.5 ug of preimmune rabbit IgG. C. Immunoprecipitation of LDB1 protein by 2.5 ug of LDB1 antibody . 12% SDS-PAGE. The immunoprecipitated LDB1 protein was detected by LDB1antibody diluted at 1:1000. EasyBlot anti-rabbit IgG (anti-rabbit IgG (HRP) -01) was used as a secondary reagent.