GTX104531

antibody from GeneTex

Targeting: LDB1 CLIM2, NLI

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX104531

Provider

GeneTex

Proper citation

GeneTex Cat#GTX104531, RRID:AB_1950805

Product name

LDB1 antibody [N2C3]

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (50 ?g of whole cell lysate) A: Mouse brain 10% SDS PAGE GTX104531 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE GTX104531 diluted at 1:1000

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

LDB1 antibody [N2C3] detects LDB1 protein by western blot analysis.A. 30 ?g 293T whole cell extract B. 30 ?g A431 whole cell extract C. 30 ?g HeLa whole cell extract D. 30 ?g HepG2 whole cell extract10 % SDS-PAGELDB1 antibody [N2C3] (GTX104531) dilution: 1:1000

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

LDB1 antibody detects LDB1 protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with LDB1 antibody (GTX104531) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

LDB1 antibody [N2C3] detects LDB1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: LDB1 protein stained by LDB1 antibody [N2C3] (GTX104531) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.

Immunoprecipitation

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

LDB1antibody immunoprecipitates LDB1 protein in IP experiments.IP Sample: 1000 ?g 293T whole cell lysate/extract A. 20 £gg 293T whole cell lysate/extractB. Control with 2.5 £gg of preimmune rabbit IgGC. Immunoprecipitation of LDB1 protein by 2.5 £gg of LDB1 antibody (GTX104531)12% SDS-PAGE The immunoprecipitated LDB1 protein was detected by LDB1antibody (GTX104531) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded U87 Xenograft, using LDB1(GTX104531) antibody at 1:100 dilution.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

LDB1 antibody [N2C3] detects LDB1 protein at nucleus on mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. LDB1 antibody [N2C3] (GTX104531) dilution: 1:500.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

LDB1 antibody [N2C3] detects LDB1 protein at nucleus on rat middle brain by immunohistochemical analysis. Sample: Paraffin-embedded rat middle brain. LDB1 antibody [N2C3] (GTX104531) dilution: 1:500.