Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- TA308742 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal antibody to LDB1 (LIM domain binding 1)
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal antibody to LDB1 (LIM domain binding 1)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- LDB1
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µl
- Concentration
- NULL
Submitted references The LIM domain binding protein 1, Ldb1, has distinct roles in Neu-induced mammary tumorigenesis.
Ahmed S, Pryce BR, Al-Zahrani KN, Sabourin LA
Biochimica et biophysica acta. Molecular cell research 2018 Nov;1865(11 Pt A):1590-1597
Biochimica et biophysica acta. Molecular cell research 2018 Nov;1865(11 Pt A):1590-1597
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- LDB1 antibody [N2C3] detects LDB1 protein by western blot analysis. A. 30 ug 293T whole cell extract. B. 30 ug A431 whole cell extract. C. 30 ug HeLa whole cell extract. D. 30 ug HepG2 whole cell extract. 10 % SDS-PAGE. LDB1 antibody [N2C3] (TA308742) dil
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Sample (50 ug of whole cell lysate). A: Mouse brain. 10% SDS PAGE. TA308742 diluted at 1:1000.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using LDB1(TA308742) antibody at 1:200 dilution.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- LDB1antibody immunoprecipitates LDB1 protein in IP experiments. IP Sample: 1000 ug 293T whole cell lysate/extract. A. 20 ug 293T whole cell lysate/extract. B. Control with 2.5 ug of preimmune rabbit IgG. C. Immunoprecipitation of LDB1 protein by 2.5 ug of
- Validation comment
- IP
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- LDB1 antibody [N2C3] detects LDB1 protein at nucleus on mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. LDB1 antibody [N2C3] (TA308742) dilution: 1:500.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- LDB1 antibody [N2C3] detects LDB1 protein at nucleus on rat middle brain by immunohistochemical analysis. Sample: Paraffin-embedded rat middle brain. LDB1 antibody [N2C3] (TA308742) dilution: 1:500.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded U87 Xenograft, using LDB1(TA308742) antibody at 1:100 dilution.
- Validation comment
- IHC