600-401-350

antibody from Rockland Immunochemicals, Inc.

Targeting: LDB1 CLIM2, NLI

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

600-401-350

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#600-401-350, RRID:AB_2137038

Product name

Anti-LDB1 (CLIM2) (RABBIT) Antibody - 600-401-350

Antibody type

Polyclonal

Vial size

100 µl

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western blot using Rockland's affinity purified anti-LDB1 antibody shows detection of LDB1 protein (arrowhead) in Jurkat whole cell lysate.   Approximately 30 µg of lysate was loaded prior to separation and transfer to nitrocellulose.  Primary antibody was used at a 1:1,800 dilution in 5% BLOTTO in PBS reacted overnight at 4°C.  The membrane was washed and reacted with a 1:20,000 dilution of DyLight™800 conjugated Gt-a-Rabbit IgG [H&L] MX (611-145-122) for 45 min at room temperature (800 nm channel, green).   Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).   Fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.  Other detection systems will yield similar results.

Validation comment

Western Blot