NBP1-31362

antibody from Novus Biologicals

Targeting: LDB1 CLIM2, NLI

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-31362

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-31362, RRID:AB_2136739

Product name

Rabbit Polyclonal LDB1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: LDB1 Antibody [NBP1-31362] - Sample: 1000 ug 293T whole cell lysate/extract A. 20 ug 293T whole cell lysate/extract, B. Control with 2. 5 ug of preimmune rabbit IgGC. Immunoprecipitation of LDB1 protein by 2. 5 ug of LDB1 antibody diluted at 1:1000.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: LDB1 Antibody [NBP1-31362] - Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE; antibody diluted at 1:1000.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: LDB1 Antibody [NBP1-31362] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with LDB1 antibody diluted by 1:1000.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: LDB1 Antibody [NBP1-31362] - Wild-type (WT) and LDB1 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with LDB1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: LDB1 Antibody [NBP1-31362] - Paraffin-embedded U87 Xenograft, using antibody at 1:100 dilution.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: LDB1 Antibody [NBP1-31362] - rat middle brain.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: LDB1 Antibody [NBP1-31362] - mouse muscle.