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- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- GTX23499 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX23499, RRID:AB_384072
- Product name
- LDB1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot using GeneTex affinity purified anti-LDB1 antibody (GTX23499) shows detection of LDB1 protein (arrowhead) in Jurkat whole cell lysate. Approximately 30 g of lysate was loaded prior to separation and transfer to nitrocellulose. Primary antibody was used at a 1:1,800 dilution in 5% BLOTTO in PBS reacted overnight at 4°C. The membrane was washed and reacted with a 1:20,000 dilution of DyLight800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot using GeneTex affinity purified anti-LDB1 antibody (GTX23499) shows detection of LDB1 protein (arrowhead) in Jurkat whole cell lysate. Approximately 30 g of lysate was loaded prior to separation and transfer to nitrocellulose. Primary antibody was used at a 1:1,800 dilution in 5% BLOTTO in PBS reacted overnight at 4¢XC. The membrane was washed and reacted with a 1:20,000 dilution of DyLight800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).
