PAB20350

antibody from Abnova Corporation

Targeting: TAF5 TAF2D, TAFII100

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB20350

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB20350, RRID:AB_10965380

Product name

TAF5 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant TAF5.

Antigen sequence

IVQEHLYIDIFDGMPRSKQQIDAMVGSLAGEAKRE
ANKSKVFFGLLKEPEIEVPLDDEDEEGENEEGKPK
KKKPKKDSIGSKSKKQDPNAPPQNRIPLPELKDSD
KLDKIMNMKETTK


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with TAF5 polyclonal antibody (Cat # PAB20350) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 with TAF5 polyclonal antibody (Cat # PAB20350) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex with TAF5 polyclonal antibody (Cat # PAB20350) shows strong nuclear and cytoplasmic positivity in neuronal cells and glial cells at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)