AF908

antibody from Novus Biologicals

Targeting: MMP8 CLG1

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF908

Provider

Novus Biologicals

Product name

Goat Polyclonal MMP-8 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human MMP-8 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse (rm) MMP-8 and recombinant rat MMP-8 is observed, and less than 1% cross-reactivity with recombinant human (rh) MMP-1, rhMMP-2, rhMMP-3, rhMMP-7, rhMMP-9, rhMMP-10, rhMMP-12, rhMMP-13, rmMMP-3, and rmMMP-9 is observed.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Redundancy of IL-1 Isoform Signaling and Its Implications for Arterial Remodeling.

Beltrami-Moreira M, Vromman A, Sukhova GK, Folco EJ, Libby P
PloS one 2016;11(3):e0152474

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Matrix-metalloproteinase-14 deficiency in bone-marrow-derived cells promotes collagen accumulation in mouse atherosclerotic plaques.

Schneider F, Sukhova GK, Aikawa M, Canner J, Gerdes N, Tang SM, Shi GP, Apte SS, Libby P
Circulation 2008 Feb 19;117(7):931-9

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

MMP-8 in Human Breast. MMP-8 was detected in immersion fixed paraffin-embedded sections of human breast array using Goat Anti-Human MMP-8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF908) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.