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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-28246 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MMP8 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HCT116.
- Concentration
- 1 mg/mL
Submitted references Matrix modification for enhancing the transport properties of the human cartilage endplate to improve disc nutrition.
Dolor A, Sampson SL, Lazar AA, Lotz JC, Szoka FC, Fields AJ
PloS one 2019;14(4):e0215218
PloS one 2019;14(4):e0215218
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using MMP8 Polyclonal Antibody (Product # PA5-28246). Sample (30 µg of whole cell lysate). Lane A: HCT116. 7.5% SDS PAGE. MMP8 Polyclonal Antibody (Product # PA5-28246) diluted at 1:10,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using MMP8 Polyclonal Antibody (Product # PA5-28246). Sample (30 µg of whole cell lysate). Lane A: HCT116. 7.5% SDS PAGE. MMP8 Polyclonal Antibody (Product # PA5-28246) diluted at 1:10,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MMP8 in methanol-fixed A431 cells using a MMP8 polyclonal antibody (Product # PA5-28246) at a 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human hepatoma, using MMP8 (Product # PA5-28246) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MMP8 Polyclonal Antibody immunoprecipitates MMP8 protein in IP experiments. IP samples: A549 whole cell extract. A. Control with 4 µg of preimmune Rabbit IgG. B. Immunoprecipitation of MMP8 protein by 4 µg MMP8 Polyclonal Antibody (Product # PA5-28246). 10 % SDS-PAGE. The immunoprecipitated MMP8 protein was detected by MMP8 Polyclonal Antibody (Product # PA5-28246) diluted at 1:500.
