Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-16498 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MMP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 500 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
Submitted references Insulin-like growth factor-independent insulin-like growth factor binding protein 3 promotes cell migration and lymph node metastasis of oral squamous cell carcinoma cells by requirement of integrin β1.
New diagnostic markers in salivary gland tumors.
Invasion-associated genes identified by gene expression profiling in extranodal natural killer/T-cell lymphoma, nasal type.
Matrix metalloproteinase, vitamin A and methylprednisolone effects on experimentally induced amyloid arthropathy.
Low microvascular density at the tumor center is related to the expression of metalloproteases and their inhibitors and with the occurrence of distant metastasis in breast carcinomas.
Host gene-encoded severe lung TB: from genes to the potential pathways.
Effect of the expression of matrix metalloproteases and their tissue inhibitors on survival of patients with resectable colorectal cancer.
Yen YC, Hsiao JR, Jiang SS, Chang JS, Wang SH, Shen YY, Chen CH, Chang IS, Chang JY, Chen YW
Oncotarget 2015 Dec 8;6(39):41837-55
Oncotarget 2015 Dec 8;6(39):41837-55
New diagnostic markers in salivary gland tumors.
Schneider S, Kloimstein P, Pammer J, Brannath W, Grasl MCh, Erovic BM
European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery 2014 Jul;271(7):1999-2007
European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery 2014 Jul;271(7):1999-2007
Invasion-associated genes identified by gene expression profiling in extranodal natural killer/T-cell lymphoma, nasal type.
Yu JB, Zhang YC, Yang QP, Wang XL, Tang Y, Zhao S, Mo XM, Liu WP
Leukemia & lymphoma 2013 Jan;54(1):90-8
Leukemia & lymphoma 2013 Jan;54(1):90-8
Matrix metalloproteinase, vitamin A and methylprednisolone effects on experimentally induced amyloid arthropathy.
Sevimli A, Yağcı A, Bülbül A
Biotechnic & histochemistry : official publication of the Biological Stain Commission 2013 Aug;88(6):336-44
Biotechnic & histochemistry : official publication of the Biological Stain Commission 2013 Aug;88(6):336-44
Low microvascular density at the tumor center is related to the expression of metalloproteases and their inhibitors and with the occurrence of distant metastasis in breast carcinomas.
Fernández-Guinea O, Álvarez-Cofiño A, Eiró N, González LO, del Casar JM, Fernandez-Garcia B, Lamelas ML, Andicoechea A, Vizoso FJ
International journal of clinical oncology 2013 Aug;18(4):629-40
International journal of clinical oncology 2013 Aug;18(4):629-40
Host gene-encoded severe lung TB: from genes to the potential pathways.
Ganachari M, Guio H, Zhao N, Flores-Villanueva PO
Genes and immunity 2012 Dec;13(8):605-20
Genes and immunity 2012 Dec;13(8):605-20
Effect of the expression of matrix metalloproteases and their tissue inhibitors on survival of patients with resectable colorectal cancer.
González L, Eiró N, González LO, Andicoechea A, Barbón E, García-Muñiz JL, Vizoso FJ
Digestive diseases and sciences 2012 Aug;57(8):2063-71
Digestive diseases and sciences 2012 Aug;57(8):2063-71
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of MMP-1 (Collagenase-1) using MMP-1 (Collagenase-1) Polyclonal Antibody (Product # PA5-16498) on HFL-1+TPA med Cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of MMP-1 (Collagenase-1) was performed using 70% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with MMP-1 (Collagenase-1) Rabbit Polyclonal Antibody (Product # 36-6300) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows a no primary antibody control. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded human placenta stained with MMP-1 antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of trophoblasts.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of MMP-1 was done on HT-29 cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with MMP-1 Rabbit Polyclonal Antibody (PA516498, red histogram) or with rabbit isotype control (pink histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Goat Anti-Rabbit Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 TB patients carrying the two-locus genotype -2518 MCP-1 GG -1607 MMP-1 2G/2G express MCP-1, MMP-1, and MMP9 We used double IHC analysis of MCP-1 or MMP-1 or MMP-9 and CD68 in paraffin-embedded diseased lung from six TB patients who underwent surgery to remove damaged tissue. We are showing representative data. A: negative control (irrelevant antibodies isotype control); B: MCP-1 (blue) and CD68 (red); C: MMP-1 (blue) and CD68 (light red); D: MMP-9 (blue) and CD68 (light red). Images were acquired at 200X total magnification. IHC analysis shows granulomas with CD68-positive cells (macrophages) expressing copious amounts of MCP-1, MMP-1, and MMP-9.