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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX100521 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX100521, RRID:AB_11169660
- Product name
- IGF1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: PC-3 B: U87-MG C: SK-N-SH 15% SDS PAGE GTX100521 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IGF1 antibody detects IGF1 protein at Golgi apparatus membrane by immunofluorescent analysis.Sample: SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: IGF1 protein stained by IGF1 antibody (GTX100521) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IGF1 antibody detects IGF1 protein at cytoplasm on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. IGF1 antibody (GTX100521) diluted at 1:500.
