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- Antibody Data
- Antigen structure
- References [16]
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- Validations
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- AF791 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse IGF-I/IGF-1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse IGF-I/IGF-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human IGF-I/IGF-1 is observed, and less than 2% cross-reactivity with recombinant mouse IGF-II/IGF-2 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q8CAR0- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Forceful mastication activates osteocytes and builds a stout jawbone.
Microglia are an essential component of the neuroprotective scar that forms after spinal cord injury.
Obesity-Activated Adipose-Derived Stromal Cells Promote Breast Cancer Growth and Invasion.
AHNAK Loss in Mice Promotes Type II Pneumocyte Hyperplasia and Lung Tumor Development.
Systemic IGF-1 gene delivery by rAAV9 improves spontaneous autoimmune peripheral polyneuropathy (SAPP).
Immunization with Bacillus Calmette-Guérin (BCG) alleviates neuroinflammation and cognitive deficits in APP/PS1 mice via the recruitment of inflammation-resolving monocytes to the brain.
Combined Effects of Androgen and Growth Hormone on Osteoblast Marker Expression in Mouse C2C12 and MC3T3-E1 Cells Induced by Bone Morphogenetic Protein.
Endothelial Cords Promote Tumor Initial Growth prior to Vascular Function through a Paracrine Mechanism.
Survival of neural stem cell grafts in the lesioned spinal cord is enhanced by a combination of treadmill locomotor training via insulin-like growth factor-1 signaling.
Viral expression of insulin-like growth factor I E-peptides increases skeletal muscle mass but at the expense of strength.
Attenuation of AD-like neuropathology by harnessing peripheral immune cells: local elevation of IL-10 and MMP-9.
Low-dose GH supplementation reduces the TLR2 and TNF-alpha expressions in visceral fat.
Role of IGF signaling in olfactory sensory map formation and axon guidance.
Microglia activated by IL-4 or IFN-gamma differentially induce neurogenesis and oligodendrogenesis from adult stem/progenitor cells.
Glatiramer acetate fights against Alzheimer's disease by inducing dendritic-like microglia expressing insulin-like growth factor 1.
Induction and blockage of oligodendrogenesis by differently activated microglia in an animal model of multiple sclerosis.
Inoue M, Ono T, Kameo Y, Sasaki F, Ono T, Adachi T, Nakashima T
Scientific reports 2019 Mar 20;9(1):4404
Scientific reports 2019 Mar 20;9(1):4404
Microglia are an essential component of the neuroprotective scar that forms after spinal cord injury.
Bellver-Landete V, Bretheau F, Mailhot B, Vallières N, Lessard M, Janelle ME, Vernoux N, Tremblay MÈ, Fuehrmann T, Shoichet MS, Lacroix S
Nature communications 2019 Jan 31;10(1):518
Nature communications 2019 Jan 31;10(1):518
Obesity-Activated Adipose-Derived Stromal Cells Promote Breast Cancer Growth and Invasion.
Hillers LE, D'Amato JV, Chamberlin T, Paderta G, Arendt LM
Neoplasia (New York, N.Y.) 2018 Nov;20(11):1161-1174
Neoplasia (New York, N.Y.) 2018 Nov;20(11):1161-1174
AHNAK Loss in Mice Promotes Type II Pneumocyte Hyperplasia and Lung Tumor Development.
Park JW, Kim IY, Choi JW, Lim HJ, Shin JH, Kim YN, Lee SH, Son Y, Sohn M, Woo JK, Jeong JH, Lee C, Bae YS, Seong JK
Molecular cancer research : MCR 2018 Aug;16(8):1287-1298
Molecular cancer research : MCR 2018 Aug;16(8):1287-1298
Systemic IGF-1 gene delivery by rAAV9 improves spontaneous autoimmune peripheral polyneuropathy (SAPP).
Gao T, Bogdanova N, Ghauri S, Zhang G, Lin J, Sheikh K
Scientific reports 2018 Apr 3;8(1):5408
Scientific reports 2018 Apr 3;8(1):5408
Immunization with Bacillus Calmette-Guérin (BCG) alleviates neuroinflammation and cognitive deficits in APP/PS1 mice via the recruitment of inflammation-resolving monocytes to the brain.
Zuo Z, Qi F, Yang J, Wang X, Wu Y, Wen Y, Yuan Q, Zou J, Guo K, Yao ZB
Neurobiology of disease 2017 May;101:27-39
Neurobiology of disease 2017 May;101:27-39
Combined Effects of Androgen and Growth Hormone on Osteoblast Marker Expression in Mouse C2C12 and MC3T3-E1 Cells Induced by Bone Morphogenetic Protein.
Kimura K, Terasaka T, Iwata N, Katsuyama T, Komatsubara M, Nagao R, Inagaki K, Otsuka F
Journal of clinical medicine 2017 Jan 5;6(1)
Journal of clinical medicine 2017 Jan 5;6(1)
Endothelial Cords Promote Tumor Initial Growth prior to Vascular Function through a Paracrine Mechanism.
Zhao C, Zhang W, Zhao Y, Yang Y, Luo H, Ji G, Dong E, Deng H, Lin S, Wei Y, Yang H
Scientific reports 2016 Jan 14;6:19404
Scientific reports 2016 Jan 14;6:19404
Survival of neural stem cell grafts in the lesioned spinal cord is enhanced by a combination of treadmill locomotor training via insulin-like growth factor-1 signaling.
Hwang DH, Shin HY, Kwon MJ, Choi JY, Ryu BY, Kim BG
The Journal of neuroscience : the official journal of the Society for Neuroscience 2014 Sep 17;34(38):12788-800
The Journal of neuroscience : the official journal of the Society for Neuroscience 2014 Sep 17;34(38):12788-800
Viral expression of insulin-like growth factor I E-peptides increases skeletal muscle mass but at the expense of strength.
Brisson BK, Spinazzola J, Park S, Barton ER
American journal of physiology. Endocrinology and metabolism 2014 Apr 15;306(8):E965-74
American journal of physiology. Endocrinology and metabolism 2014 Apr 15;306(8):E965-74
Attenuation of AD-like neuropathology by harnessing peripheral immune cells: local elevation of IL-10 and MMP-9.
Koronyo-Hamaoui M, Ko MK, Koronyo Y, Azoulay D, Seksenyan A, Kunis G, Pham M, Bakhsheshian J, Rogeri P, Black KL, Farkas DL, Schwartz M
Journal of neurochemistry 2009 Dec;111(6):1409-24
Journal of neurochemistry 2009 Dec;111(6):1409-24
Low-dose GH supplementation reduces the TLR2 and TNF-alpha expressions in visceral fat.
Kubota Y, Unoki H, Bujo H, Rikihisa N, Udagawa A, Yoshimoto S, Ichinose M, Saito Y
Biochemical and biophysical research communications 2008 Mar 28;368(1):81-7
Biochemical and biophysical research communications 2008 Mar 28;368(1):81-7
Role of IGF signaling in olfactory sensory map formation and axon guidance.
Scolnick JA, Cui K, Duggan CD, Xuan S, Yuan XB, Efstratiadis A, Ngai J
Neuron 2008 Mar 27;57(6):847-57
Neuron 2008 Mar 27;57(6):847-57
Microglia activated by IL-4 or IFN-gamma differentially induce neurogenesis and oligodendrogenesis from adult stem/progenitor cells.
Butovsky O, Ziv Y, Schwartz A, Landa G, Talpalar AE, Pluchino S, Martino G, Schwartz M
Molecular and cellular neurosciences 2006 Jan;31(1):149-60
Molecular and cellular neurosciences 2006 Jan;31(1):149-60
Glatiramer acetate fights against Alzheimer's disease by inducing dendritic-like microglia expressing insulin-like growth factor 1.
Butovsky O, Koronyo-Hamaoui M, Kunis G, Ophir E, Landa G, Cohen H, Schwartz M
Proceedings of the National Academy of Sciences of the United States of America 2006 Aug 1;103(31):11784-9
Proceedings of the National Academy of Sciences of the United States of America 2006 Aug 1;103(31):11784-9
Induction and blockage of oligodendrogenesis by differently activated microglia in an animal model of multiple sclerosis.
Butovsky O, Landa G, Kunis G, Ziv Y, Avidan H, Greenberg N, Schwartz A, Smirnov I, Pollack A, Jung S, Schwartz M
The Journal of clinical investigation 2006 Apr;116(4):905-15
The Journal of clinical investigation 2006 Apr;116(4):905-15
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IGF-I/IGF-1 in Mouse Embryo. IGF-I/IGF-1 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF791) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to developing brain and muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IGF-I/IGF-1 and Neutralization by Mouse IGF-I/IGF-1 Antibody. Recombinant Mouse IGF-I/IGF-1 (Catalog # 791-MG) stimulates proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IGF-I/IGF-1 (15 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Mouse IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF791). The ND50 is typically 0.2-1.0 µg/mL.
