H00008189-M03

antibody from Abnova Corporation

Targeting: SYMPK SPK, SYM

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00008189-M03

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00008189-M03, RRID:AB_519080

Product name

SYMPK monoclonal antibody (M03), clone 4C2

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant SYMPK.

Antigen sequence

MASGSGDSVTRRSVASQFFTQEEGPGIDGMTTSER
VVDLLNQAALITNDSKITVLKQVQELIINKDPTLL
DNFLDEIIAFQADKSIEVRKFVIGFIEEACKRDIE
LLLKLIANLNMLLRDENVNVVKKAILTMTQLYKVA
LQWMVKSRVISELQEACWDMVSAMAGDIILLLDSD
NDGIRTHAIKFVEGLIVTLSPRMADSEIPRRQEHD
ISLDRIPRDHPYIQYNVLWEEGKAALEQLLKFMVH
PAISSINLTTALGSLANIARQRPMFMSEVIQAYET
LHANLPPTLAKSQVSSVRKNLKLHLLSVLKHPASL
EFQAQITTLLVDLGTPQAEIARNMPSSKDTRKRPR
DDSDSTLKKMKLEPNLGEDDEDKDLEPGPSGTSKA
SAQISGQSDTDITAEFLQPLLTPDNVANLVLISMV
YLPEAMPASFQAIYTPVESAGTEAQIKHLARLMAT
QMTAAGLGPGVEQTKQCKEEPKEEKVVKTESVLIK
RRLSAQGQAISVVGSLSSMSPLEEEAPQAKRRPEP
IIPVTQPR


Isotype

IgG

Antibody clone number

4C2

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of SYMPK expression in transfected 293T cell line by SYMPK monoclonal antibody (M03), clone 4C2.Lane 1: SYMPK transfected lysate(58.9 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged SYMPK is approximately 0.03ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to SYMPK on HeLa cell. [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to SYMPK on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma. [antibody concentration 3 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol