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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [8]
- Other assay [1]
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Validation data
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- Product number
- 11519-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Symplekin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MTQLYKVAL QWMVKSRVIS ELQEACWDMV SAMAGDIILL LDSDNDGIRT HAIKFVEGLI VTLSPRMADS EIPRRQEHDI SLDRIPRDHP YIQYNVLWEE GKAALEQLLK FMVHPAISSI NLTTALGSLA NIARQRPMFM SEVIQAYETL HANLPPTLAK SQVSSVRKNL KLHLLSVLKH PASLEFQAQI TTLLVDLGTP QAEIARNMPS SKDTRKRPRD DSDSTLKKMK LEPNLGEDDE DKDLEPGPSG TSKASAQISG QSDTDITAEF LQPLLTPDNV ANLVLISMVY LPEAMPASFQ AIYTPVESAG TEAQIKHLAR LMATQMTAAG LGPGVEQTKQ CKEEPKEEKV VKTESVLIKR RL (118-478 aa encoded by BC006567)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.3 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- K-562 cells were subjected to SDS PAGE followed by western blot with 11519-1-AP (Symplekin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MCF7 cells were subjected to SDS PAGE followed by western blot with 11519-1-AP (Symplekin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis using 11519-1-AP (Symplekin antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis using 11519-1-AP (Symplekin antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon cancer using 11519-1-AP (Symplekin antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon cancer using 11519-1-AP (Symplekin antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human placenta using 11519-1-AP (Symplekin antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human placenta using 11519-1-AP (Symplekin antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver using 11519-1-AP (Symplekin antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver using 11519-1-AP (Symplekin antibody) at dilution of 1:50 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-Symplekin (IP:11519-1-AP, 4ug; Detection:11519-1-AP 1:500) with K-562 cells lysate 3600ug.
