Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NB100-74590 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-74590, RRID:AB_1049777
- Product name
- Rabbit Polyclonal Symplekin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Symplekin Antibody [NB100-74590] - Detection of Human Symplekin by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Symplekin antibody NB100-74590 used for IP at 3 mcg/mg lysate. Symplekin was also immunoprecipitated by rabbit anti-Symplekin antibodies NB100-74591 and NB100-74592, which recognize downstream epitopes. Detection: Chemiluminescence with an exposure time of 1 second.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Symplekin Antibody [NB100-74590] - Detection of human Symplekin by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Symplekin antibody NB100-74590 (lot 2) used for IP at 6 ug per reaction. Symplekin was also immunoprecipitated by a previous lot of this antibody (lot 1). For blotting immunoprecipitated Symplekin, NB100-74590 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Symplekin Antibody [NB100-74590] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- Symplekin used at a dilution of 1:1,000 (1ug/ml). Detection: DAB