H00000429-M01

antibody from Abnova Corporation

Targeting: ASCL1 ASH1, bHLHa46, HASH1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00000429-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00000429-M01, RRID:AB_529967

Product name

ASCL1 monoclonal antibody (M01), clone 7E11

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant ASCL1.

Antigen sequence

GFATLREHVPNGAANKKMSKVETLRSAVEYIRALQ
QLLDEHDAVSAAFQAGVLSPTISPNYSNDLNSMAG
SPVSSYSSDEGSYDPLSPEEQELLDFTNWF


Isotype

IgG

Antibody clone number

7E11

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of ASCL1 expression in transfected 293T cell line by ASCL1 monoclonal antibody (M01), clone 7E11.Lane 1: ASCL1 transfected lysate(25.5 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged ASCL1 is approximately 1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol