600-101-MH4

antibody from Invitrogen Antibodies

Targeting: ASCL1 ASH1, bHLHa46, HASH1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

600-101-MH4

Provider

Invitrogen Antibodies

Product name

Mash1/Ascl1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot of Goat Anti-Mash1/Ascl1 Antibody. Lane 1: Opal Pre-Stained Molecular Weight Marker (p/n MB-210-0500). Lane 2: MASH1/ASCL1 Human recombinant protein C-MYC/DDK-tagged (50ng) [+]. Primary Antibody: Anti-Mash1/Ascl1 at 1:1000 overnight at 2-8°C. Secondary Antibody: Donkey Fab2 Anti-Goat H&L mx8 HRP at 1:5000 at RT for 1hr. Blocking Buffer: BlockOut® - Universal Blocking Buffer for Western Blotting (p/n MB-073). Expect: 24.7kDa, ~37kDa recombinant.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot of Goat Anti-Mash1/Ascl1 Antibody. Opal Pre-Stained Molecular Weight Marker (p/n MB-210-0500). Lane 1: Mouse Pancreas (35µg) (p/n W10-000-T023) [-]. Lane 2: SH-SY5Y whole cell lysate (35µg) [+]. Primary Antibody: Anti-Mash1/Ascl1 at 1:1000 overnight at 2-8°C. Secondary Antibody: Rabbit Anti-Goat IgG HRP (p/n 605-4313) at 1:5000 at RT for 1hr. Blocking Buffer: BlockOut® - Universal Blocking Buffer for Western Blotting (p/n MB-073). Expect: 24.7kDa, observed ~37kDa.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of Goat Anti-Mash1/Ascl1 Antibody. Tissue: Human Thyroid Papillary Carcinoma Tissue. Fixative: none. Antigen Retrieval: HEIR pH6. Primary Antibody: Anti-Mash1/Ascl1 at 1:1,000. Counterstain: Hematoxylin. The Abs developed intense nucleus staining in human thyroid papillary cancer tissue.