Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- ELISA [1]
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Validation data
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- Product number
- MAB913 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Pro MMP-13 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Pro MMP-13 in direct ELISAs and Western blots. Does not detect active recombinant human MMP-13. In Western blots, no cross-reactivity with recombinant human MMP-1, -2, -3, -7, -8, -9, -10, or -12 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P45452
- Isotype
- IgG
- Antibody clone number
- 87518
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Detection of changes in articular cartilage proteoglycan by T(1rho) magnetic resonance imaging.
Wheaton AJ, Dodge GR, Borthakur A, Kneeland JB, Schumacher HR, Reddy R
Journal of orthopaedic research : official publication of the Orthopaedic Research Society 2005 Jan;23(1):102-8
Journal of orthopaedic research : official publication of the Orthopaedic Research Society 2005 Jan;23(1):102-8
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Human Pro- MMP-13 ELISA Standard Curve. Recombinant Human Pro-MMP-13 protein was serially diluted 2-fold and captured by Mouse Anti-Human MMP-13 Monoclonal Antibody (Catalog # MAB5111) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Pro MMP-13 Monoclonal Antibody (Catalog # MAB913) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).