H00057016-M01

AKR1B10 antibody from Abnova Corporation

AKR1B11, AKR1B12, ALDRLn, ARL-1, ARL1, HIS, HSI

Western blot

Supportive data in Antibodypedia

ELISA

Supportive data in Antibodypedia

Immunocytochemistry

Supportive data in Antibodypedia

Immunohistochemistry

Supportive data in Antibodypedia

Provider product page for H00057016-M01

Antibody Data

Product number

H00057016-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00057016-M01, RRID:AB_509242

Product name

AKR1B10 monoclonal antibody (M01), clone 1A6

Provider product page

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant AKR1B10.

Antigen sequence

VSKLWPTFFERPLVRKAFEKTLKDLKLSYLDVYLI
HWPQGFKSGDDLFPKDDKGNAIGGKATFLDAWE


Isotype

IgG

Antibody clone number

1A6

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Quantification of the host response proteome after herpes simplex virus type 1 infection.
Berard AR, Coombs KM, Severini A
Journal of proteome research 2015 May 1;14(5):2121-42

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A therapeutic method for the direct reprogramming of human liver cancer cells with only chemicals.
Moriguchi H, Zhang Y, Mihara M, Sato C
Scientific reports 2012;2:280

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An integrated functional genomics approach identifies the regulatory network directed by brachyury (T) in chordoma.
Nelson AC, Pillay N, Henderson S, Presneau N, Tirabosco R, Halai D, Berisha F, Flicek P, Stemple DL, Stern CD, Wardle FC, Flanagan AM
The Journal of pathology 2012 Nov;228(3):274-85

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Overexpression and oncogenic function of aldo-keto reductase family 1B10 (AKR1B10) in pancreatic carcinoma.
Chung YT, Matkowskyj KA, Li H, Bai H, Zhang W, Tsao MS, Liao J, Yang GY
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2012 May;25(5):758-66

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Combined functional genome survey of therapeutic targets for hepatocellular carcinoma.
Satow R, Shitashige M, Kanai Y, Takeshita F, Ojima H, Jigami T, Honda K, Kosuge T, Ochiya T, Hirohashi S, Yamada T
Clinical cancer research : an official journal of the American Association for Cancer Research 2010 May 1;16(9):2518-28

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Naturally occurring variants of human aldo-keto reductases with reduced in vitro metabolism of daunorubicin and doxorubicin.
Bains OS, Grigliatti TA, Reid RE, Riggs KW
The Journal of pharmacology and experimental therapeutics 2010 Dec;335(3):533-45

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

AKR1B10 monoclonal antibody (M01), clone 1A6 Western Blot analysis of AKR1B10 expression in HepG2 ( Cat # L019V1 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of AKR1B10 expression in transfected 293T cell line by AKR1B10 monoclonal antibody (M01), clone 1A6.Lane 1: AKR1B10 transfected lysate(36 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged AKR1B10 is approximately 0.03ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to AKR1B10 on HeLa cell. [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to AKR1B10 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol