PA5-22036

antibody from Invitrogen Antibodies

Targeting: AKR1B10 AKR1B11, AKR1B12, ALDRLn, ARL-1, ARL1, HIS, HSI

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-22036

Provider

Invitrogen Antibodies

Product name

AKR1B10 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Recommended positive controls: Molt-4, Raji, mouse colon, rat colon, Jurkat, NCI-H929.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of AKR1B10 using 30 µg of A) MOLT4 and B) Raji lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with an AKR1B10 polyclonal antibody (Product # PA5-22036) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

AKR1B10 Polyclonal Antibody detects AKR1B10 protein by western blot analysis. A. 50 µg mouse colon lysate/extract.10% SDS-PAGE. AKR1B10 Polyclonal Antibody (Product # PA5-22036) dilution: 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

AKR1B10 Polyclonal Antibody detects AKR1B10 protein by western blot analysis. A. 50 µg rat colon lysate/extract.10% SDS-PAGE. AKR1B10 Polyclonal Antibody (Product # PA5-22036) dilution: 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

AKR1B10 Polyclonal Antibody detects AKR1B10 protein by Western blot analysis. Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with AKR1B10 Polyclonal Antibody (Product # PA5-22036) diluted by 1:2,500.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of AKR1B10 in paraformaldehyde-fixed HeLa cells using an AKR1B10 polyclonal antibody (Product # PA5-22036) at a 1:200 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using AKR1B10 antibody (Product # PA5-22036) at 1:200 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using AKR1B10 (Product # PA5-22036) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.