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LearnPA5-22036
antibody from Invitrogen Antibodies
Targeting: AKR1B10
AKR1B11, AKR1B12, ALDRLn, ARL-1, ARL1, HIS, HSI
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-22036 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- AKR1B10 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of AKR1B10 using 30 µg of A) MOLT4 and B) Raji lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with an AKR1B10 polyclonal antibody (Product # PA5-22036) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- AKR1B10 Polyclonal Antibody detects AKR1B10 protein by western blot analysis. A. 50 µg mouse colon lysate/extract.10% SDS-PAGE. AKR1B10 Polyclonal Antibody (Product # PA5-22036) dilution: 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- AKR1B10 Polyclonal Antibody detects AKR1B10 protein by western blot analysis. A. 50 µg rat colon lysate/extract.10% SDS-PAGE. AKR1B10 Polyclonal Antibody (Product # PA5-22036) dilution: 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- AKR1B10 Polyclonal Antibody detects AKR1B10 protein by Western blot analysis. Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with AKR1B10 Polyclonal Antibody (Product # PA5-22036) diluted by 1:2,500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of AKR1B10 in paraformaldehyde-fixed HeLa cells using an AKR1B10 polyclonal antibody (Product # PA5-22036) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using AKR1B10 antibody (Product # PA5-22036) at 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using AKR1B10 (Product # PA5-22036) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
