PA5-30773

antibody from Invitrogen Antibodies

Targeting: AKR1B10 AKR1B11, AKR1B12, ALDRLn, ARL-1, ARL1, HIS, HSI

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-30773

Provider

Invitrogen Antibodies

Product name

AKR1B10 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: HeLa, HCT116.

Concentration

0.34 mg/mL

References

Submitted references

Single-cell RNA profiling of Plasmodium vivax-infected hepatocytes reveals parasite- and host- specific transcriptomic signatures and therapeutic targets.

Ruberto AA, Maher SP, Vantaux A, Joyner CJ, Bourke C, Balan B, Jex A, Mueller I, Witkowski B, Kyle DE
Frontiers in cellular and infection microbiology 2022;12:986314

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using AKR1B10 Polyclonal Antibody (Product # PA5-30773). Sample (30 µg of whole cell lysate). Lane A: HCT116. 10% SDS PAGE. AKR1B10 Polyclonal Antibody (Product # PA5-30773) diluted at 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of AKR1B10 in methanol-fixed HeLa cells using an AKR1B10 polyclonal antibody (Product # PA5-30773) at a 1:200 dilution.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Analysis of host transcriptional signatures in P. vivax infected hepatocytes. (A) Volcano plots showing changes in gene expression in infected hepatocytes versus non infected (Wilcoxon rank sum, Bonferroni adjusted p value < 0.05). Positive fold change (FC) values represent genes with greater expression, and negative FC values represent genes with decreased expression. Dashed horizontal red lines: Bonferroni adjusted p value = 0.05; dashed vertical red lines: log 2 FC = 0.5. (B) Summary of differential gene expression data shown in the volcano plots and of the enrichment analyses. Hep: hepatocyte. (C) Enrichment map of cellular processes and pathways associated with hepatocyte infection with P. vivax schizonts. (D) Enrichment map of cellular processes and pathways associated with hepatocyte infection with P. vivax hypnozoites. For the maps depicted in panels C and D, node size is proportional to the number of genes identified in each gene set (minimum 3 genes/gene set); each node represents a distinct biological process or pathway derived from gene with decreased expression (red) or increased expression (blue) versus non-infected cells; and edges (blue lines) represent the number of genes overlapping between two processes or pathways. (E) alphaRepresentative confocal image of P. vivax parasites on day 12 post-infection of hepatocytes (left). Cells were stained with DAPI (blue), Pv UIS4 (green), and Hs AKR1B10 (red). White bar represents 100um. Violin plot displaying the distr