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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AP50734PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP50734PU-N, RRID:AB_11144357
- Product name
- anti Caspase-5 (Center)
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 146-175 amino acids from the Central region of human CASP5
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- CASP5 Antibody (Center) (Cat. #AP50734PU-N) western blot analysis in MDA-MB231 cell line lysates (35µg/lane).This demonstrates the CASP5 antibody detected the CASP5 protein (arrow).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- CASP5 Antibody (Center) (Cat. #AP50734PU-N) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CASP5 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- CASP5 Antibody (Center) (Cat. #AP50734PU-N) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
