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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- H00009442-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00009442-M01, RRID:AB_538003
- Product name
- Mouse Monoclonal MED27 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. CRSP8 - cofactor required for Sp1 transcriptional activation, subunit 8, 34kDa
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.05 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MED27 Antibody (8B8) [H00009442-M01] - Analysis of CRSP8 expression in transfected 293T cell line by CRSP8 monoclonal antibody (M01), clone 8B8.Lane 1: CRSP8 transfected lysate(35.4 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MED27 Antibody (8B8) [H00009442-M01] - CRSP8 monoclonal antibody (M01), clone 8B8 Analysis of CRSP8 expression in Hela S3 NE.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: MED27 Antibody (8B8) [H00009442-M01] - Detection limit for recombinant GST tagged MED27 is 0.3 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MED27 Antibody (8B8) [H00009442-M01] - Analysis of monoclonal antibody to CRSP8 on formalin-fixed paraffin-embedded human colon. Antibody concentration 0.3 ug/ml.
