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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PA5-50575 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SPATA20 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- The antibody detects endogenous levels of total SPATA20 protein.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 2.8 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SPATA20 was performed by loading (from left to right): A549 and lncap cell, human seminoma tissue lysates (60µg) on to a 10% SDS-PAGE gel. Proteins were transferred to a membrane and the membrane was probed with a SPATA20 antibody (Product # PA5-50575) at a 1/1000 dilution for 5 minutes, followed by a secondary antibody at 1/8000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of SPATA20 in paraffin embedded Human esophagus cancer tissue using SPATA20 Polyclonal Antibody (Product # PA5-50575) at a 1:70 dilution.
