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LearnPA5-48383
antibody from Invitrogen Antibodies
Targeting: POLR1B
FLJ10816, FLJ21921, RPA135, RPA2, Rpo1-2
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- PA5-48383 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- POLR1B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Predicted to react with rat based on sequence homology.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.3 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Long non-coding RNA ZFAS1 promotes colorectal cancer tumorigenesis and development through DDX21-POLR1B regulatory axis.
Wang X, Wu Z, Qin W, Sun T, Lu S, Li Y, Wang Y, Hu X, Xu D, Wu Y, Chen Q, Yao W, Liu M, Wei M, Wu H
Aging 2020 Nov 16;12(22):22656-22687
Aging 2020 Nov 16;12(22):22656-22687
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of POLR1B in Hela cell line lysates (35 µg/lane). Lysates were probed with a POLR1B Antibody (N-term) (Product # PA5-48383).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of POLR1B in formalin fixed and paraffin embedded human stomach tissue. Samples were probed with a POLR1B Antibody (N-term) (Product # PA5-48383) followed by peroxidase conjugation of the secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of POLR1B in Hela cells (right histogram) compared to a negative control cell (left histogram). Samples were incubated with a POLR1B Antibody (N-term) (Product # PA5-48383) followed by incubation with a FITC-conjugated goat-anti-rabbit secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6 DDX21-PLOR1B signaling axis regulated by lncRNA ZFAS1 in CRC cells and tissues. ( A and B ) The mRNA expression of POLR1B after overexpression or silencing lncRNA ZFAS1 in SW620 and SW480 cells by RT-PCR ( A ) and qPCR assay ( B ). ( C and D ) The expression levels of POLR1B after interfering DDX21 expression in both SW620 and SW480 cells detected by western blot ( C ) and qPCR assay ( D ). ( E and F ) Rescue experiments determining the POLR1B mRNA and protein expression levels treated by silencing of lncRNA ZFAS1 and overexpression of DDX21 in SW620 and SW480 cells assayed by qPCR assay ( E ) and western blot assay ( F ). ( G ) Representative analysis results of POLR1B expression in paired CRC tissues and controls assayed by RT-PCR and qPCR method. 5 representative data was shown. ( H ) Representative IHC imagines and Violin charts displaying the POLR1B protein expression based on the CRC patient tissues and matched tumor-adjacent controls ( n = 157). The bar represents 100mum. ( I ) Linear regression analysis illustrating a positive correlation between the expression of POLR1B and LncRNA ZFAS1 or DDX21. ( J and K ) Stratified Kaplan-Meier plot illustrating the impact of POLR1B high/low expression on the DFS and OS upon those patients with DDX21 high expression ( J ) or with ZFAS1 high expression ( K ). Data were shown as mean +- s.d. Two-tailed Student's t -tests were used. * P
