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LearnH00084172-M10
antibody from Invitrogen Antibodies
Targeting: POLR1B
FLJ10816, FLJ21921, RPA135, RPA2, Rpo1-2
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00084172-M10 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- POLR1B Monoclonal Antibody (4H6)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Sequence of this protein is as follows: GEMLKAAGYN FYGTERLYSG ISGLELEADI FIGVVYYQRL RHMVSDKFQV RTTGARDRVT NQPIGGRNVQ GGIRFGEMER DALLAHGTSF LLHDRLFNCS DRSVAHVCVK
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 4H6
- Vial size
- 100 µg
- Concentration
- See Label
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of POLR1B expression in transfected 293T cell line by POLR1B monoclonal antibody (M10), clone 4H6.Lane 1: POLR1B transfected lysate (Predicted MW: 128.2 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged POLR1B is 3 ng/mL as a capture antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to POLR1B on HeLa cell. Antibody concentration is 10 µg/mL.
