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LearnAP53382PU-N
antibody from Acris Antibodies GmbH
Targeting: POLR1B
FLJ10816, FLJ21921, RPA135, RPA2, Rpo1-2
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AP53382PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP53382PU-N, RRID:AB_11147687
- Product name
- anti POLR1B (N-term)
- Antibody type
- Polyclonal
- Antigen
- conjugated synthetic peptide between 88-117 amino acids from the N-terminal region of human POLR1B
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of POLR1B Antibody (N-term) Cat.-No AP53382PU-NÂ in Hela cell line lysates (35ug/lane). This demonstrates the POLR1B antibody detected the POLR1B protein (arrow).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin fixed and paraffin embedded human stomach tissue reacted with POLR1B Antibody (N-term) Cat.-No AP53382PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Hela cells using POLR1B Antibody (N-term) Cat.-No AP53382PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
