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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-52220 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- POLR3K Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: PGCGNGLIVE EGQRCHRFAC NTCPYVHNIT RKVTNRKYPK LKEVDDVLGG AAAWENVDST AESCPKCEHP RAYFMQLQTR SADEPMTTFY KCCNAQCGHR WRD
- Concentration
- 0.05 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of POLR3K in control (vector only transfected HEK293T lysate) and POLR3K over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a POLR3K Polyclonal Antibody (Product # PA5-52220).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of POLR3K in human cell line U-251 MG shows positivity in nucleus but excluded from the nucleoli. Samples were probed using a POLR3K Polyclonal Antibody (Product # PA5-52220).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of POLR3K in human testis using a POLR3K Polyclonal Antibody (Product # PA5-52220) shows strong nuclear positivity in cells of seminiferous ducts.
