Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- APR-068-25UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Prostacyclin Receptor (PTGIR) (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Reconstitution: 1 X 25 µL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 25 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human Jurkat T-cell leukemia cell (lanes 1 and 4), human THP-1 monocytic leukemia cell (lanes 2 and 5) and human MEG-01 megakaryoblastic leukemia cell (lanes 3 and 6) lysates: - 1-3. Anti-Prostacyclin Receptor (PTGIR) (extracellular) Antibody (#APR-068), (1:200).4-6. Anti-Prostacyclin Receptor (PTGIR) (extracellular) Antibody , preincubated with Prostacyclin Receptor/PTGIR (extracellular) Blocking Peptide (#BLP-PR068).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of mouse heart membranes (lanes 1 and 4), mouse lung lysate (lanes 2 and 5) and rat lung membranes (lanes 3 and 6): - 1-3. Anti-Prostacyclin Receptor (PTGIR) (extracellular) Antibody (#APR-068), (1:400).4-6. Anti-Prostacyclin Receptor (PTGIR) (extracellular) Antibody , preincubated with Prostacyclin Receptor/PTGIR (extracellular) Blocking Peptide (#BLP-PR068).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human Jurkat T-cell leukemia cell (lanes 1 and 4), human THP-1 monocytic leukemia cell (lanes 2 and 5) and human MEG-01 megakaryoblastic leukemia cell (lanes 3 and 6) lysates: - 1-3. Anti-Prostacyclin Receptor (PTGIR) (extracellular) Antibody (#APR-068), (1:200).4-6. Anti-Prostacyclin Receptor (PTGIR) (extracellular) Antibody , preincubated with Prostacyclin Receptor/PTGIR (extracellular) Blocking Peptide (#BLP-PR068).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cell surface detection of PTGIR by indirect flow cytometry in live intact human MEG-01 megakaryoblastic leukemia cells: - (black line) cells. (red) Cells + goat- Anti-rabbit-FITC. (green) Cells + Anti-Prostacyclin Receptor (PTGIR) (extracellular) Antibody (#APR-068), (5μg) + goat- Anti-rabbit-FITC.