APR-068-25UL

antibody from Invitrogen Antibodies

Targeting: PTGIR IP

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

APR-068-25UL

Provider

Invitrogen Antibodies

Product name

Prostacyclin Receptor (PTGIR) (extracellular) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

25 µL

Concentration

0.8 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of human Jurkat T-cell leukemia cell (lanes 1 and 4), human THP-1 monocytic leukemia cell (lanes 2 and 5) and human MEG-01 megakaryoblastic leukemia cell (lanes 3 and 6) lysates: - 1-3. Anti-Prostacyclin Receptor (PTGIR) (extracellular) Antibody (#APR-068), (1:200).4-6. Anti-Prostacyclin Receptor (PTGIR) (extracellular) Antibody , preincubated with Prostacyclin Receptor/PTGIR (extracellular) Blocking Peptide (#BLP-PR068).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of mouse heart membranes (lanes 1 and 4), mouse lung lysate (lanes 2 and 5) and rat lung membranes (lanes 3 and 6): - 1-3. Anti-Prostacyclin Receptor (PTGIR) (extracellular) Antibody (#APR-068), (1:400).4-6. Anti-Prostacyclin Receptor (PTGIR) (extracellular) Antibody , preincubated with Prostacyclin Receptor/PTGIR (extracellular) Blocking Peptide (#BLP-PR068).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of human Jurkat T-cell leukemia cell (lanes 1 and 4), human THP-1 monocytic leukemia cell (lanes 2 and 5) and human MEG-01 megakaryoblastic leukemia cell (lanes 3 and 6) lysates: - 1-3. Anti-Prostacyclin Receptor (PTGIR) (extracellular) Antibody (#APR-068), (1:200).4-6. Anti-Prostacyclin Receptor (PTGIR) (extracellular) Antibody , preincubated with Prostacyclin Receptor/PTGIR (extracellular) Blocking Peptide (#BLP-PR068).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Cell surface detection of PTGIR by indirect flow cytometry in live intact human MEG-01 megakaryoblastic leukemia cells: - (black line) cells. (red) Cells + goat- Anti-rabbit-FITC. (green) Cells + Anti-Prostacyclin Receptor (PTGIR) (extracellular) Antibody (#APR-068), (5μg) + goat- Anti-rabbit-FITC.