TA324538

antibody from OriGene

Targeting: NFIA KIAA1439, NFI-L

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA324538

Provider

OriGene

Product name

Rabbit polyclonal NFIA Antibody (C-term)

Antibody type

Polyclonal

Description

Rabbit polyclonal NFIA Antibody (C-term)

Host

Rabbit

Conjugate

Unconjugated

Epitope

NFIA

Isotype

IgG

Antibody clone number

NULL

Vial size

400 µl

Concentration

0.45 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

NFIA Antibody (C-term) (Cat. #TA324538) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the NFIA antibody detected the NFIA protein (arrow).

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

IF image of Hela cell stained with NFIA Antibody (C-term)(Cat#TA324538).Hela cells were incubated with NFIA primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) . NFIA immunoreactivity is localized to Nucleus significantly.

Validation comment

IF